bmscs complete medium cat. no. cm-r131  (Procell Inc)

Journal: Bioactive Materials

Article Title: Fused exosomal targeted therapy in periprosthetic osteolysis through regulation of bone metabolic homeostasis

doi: 10.1016/j.bioactmat.2025.04.006

Figure Lengend Snippet: Regulation of osteoblast differentiation by f-exo in vitro (A, E-F) Osteogenesis of BMSC induced by f-exo in vitro assessed by ARS, sirius red staining, ALP staining, and their quantifications. Scale bar 3 mm, 400 μm (enlarged) (B, D) the intracellular collagen production assessed by immunofluorescence staining of collagen 1α (Col1a) and quantitative analysis of fluorescence intensity. Scale bar, 200 μm (C, G-H) Osteogenic-related protein analyzed by western blotting, and its relative quantifications (The data in the figures represent the averages ± SD, n = 3 samples per group, ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001).

Article Snippet: DMEM medium(CYTOCH, Shanghai, China), RPMI 1640medium (BioChannel Biological Technology Co., Ltd.); Fetal Bovine serum (Gibco, USA); Exosome-depleted Fetal Bovine serum (Umibio (Shanghai) Co., Ltd.); Penicillin-Streptomycin Liquid (BDBIO HangZhou China); Cell Freezing Medium (A401; Witcel, Shanghai, China); Trypsin(HAKATA); PBS, TBST (ABclonal Technology, WuHan, China); Cell Plasma Membrane Staining Kit with DiI, DiO, Reactive Oxygen Species assay kit, 4 %PFA, Alkaline Phophatase Stain Kit (Beyotime, China); Complete Medium For BMSC Culture Osteogenic Differentiation (OriCell®, from Cyagen Biosciences(Guangzhou)Inc.); Goat Serum Albumin (Biodragon, AbBox, China); TRAP Activity Assay, KitModified Sirius Red Stain Kit (Beijing Solarbio Science & Technology Co., Ltd.); FITC Phalloidin, DiR Iodide (Yeasen Biotechnology (Shanghai) Co., Ltd.); BCA Protein Quantification Kit (Smar-lifesciences, SLR01201); SDS-PAGE Loading Buffer (Swiss Affinibody LifeScience AG); Titanium nanopowder (Aladdin, Shanghai); Anti-GAPDH antibody (Abcam, UK), Anti-CD63 antibody (Abcam, UK), Anti-TSG101 antibody (Abcam, UK), Anti-Calnexin antibody (Abcam, UK), Anti-col1a antibody (Abcam, UK), Anti-sp7 antibody (Abcam, UK), Anti-runx2 antibody (Abcam, UK), Anti-CTSK antibody (Abcam, UK), Anti-NFATc-1 antibody (Abcam, UK), Anti-TRAP antibody (Abcam, UK), Anti-EMCN antibody (Proteintech, China), Anti-HIF1α antibody (Abcam, UK), Anti-CD31 antibody (Abcam, UK), Anti-VEGF antibody (Abcam, UK).

Techniques: In Vitro, Staining, Immunofluorescence, Fluorescence, Western Blot

Journal: Biomaterials Research

Article Title: Repair of Osteoporotic Bone Defects in Rats via the Sirtuin 1-Wnt/β-catenin Signaling Pathway by Novel Icariin/Porous Magnesium Alloy Scaffolds

doi: 10.34133/bmr.0090

Figure Lengend Snippet: Experiments on proliferation, senescence, migration, and osteogenic differentiation of BMSCs. (A) OD values of BMSCs in the control group, ICA group, Mg 2+ group, and ICA+Mg 2+ group on the first day and fourth day of cultivation. (*, P < 0.05; **, P < 0.01). (B) β-Galactosidase staining of the effect of costimulators ICA and Mg 2+ on the aging of BMSCs. (C) Microscopic observation of the effect of costimulators ICA and Mg 2+ on the migration capacity of BMSCs. (D) ALP staining and alizarin red-S (ARS) staining for analyzing the effect of costimulators ICA and Mg 2+ on the osteogenic differentiation of BMSCs.

Article Snippet: ICA and magnesium chloride were added separately to the complete medium of BMSCs (Cyagen, China).

Techniques: Migration, Control, Staining

Journal: Biomaterials Research

Article Title: Repair of Osteoporotic Bone Defects in Rats via the Sirtuin 1-Wnt/β-catenin Signaling Pathway by Novel Icariin/Porous Magnesium Alloy Scaffolds

doi: 10.34133/bmr.0090

Figure Lengend Snippet: ICA and Mg 2+ costimulate the mRNA expression of proliferation-related genes in rat BMSCs via the SIRT1-Wnt/β-catenin signaling pathway. (A) SIRT1. (B) Wnt1a. (C) Wnt3a. (D) Wnt5a. (E) β-catenin. (F) GSK3β. (G) GM-CSF. *, P < 0.05; **, P < 0.01.

Article Snippet: ICA and magnesium chloride were added separately to the complete medium of BMSCs (Cyagen, China).

Techniques: Expressing

Journal: Biomaterials Research

Article Title: Repair of Osteoporotic Bone Defects in Rats via the Sirtuin 1-Wnt/β-catenin Signaling Pathway by Novel Icariin/Porous Magnesium Alloy Scaffolds

doi: 10.34133/bmr.0090

Figure Lengend Snippet: ICA and Mg 2+ costimulate the mRNA expression of factors related to osteogenic differentiation of rat BMSCs via the SIRT1-Wnt/β-catenin signaling pathway. (A) SIRT1. (B) Wnt5a. (C) β-Catenin. (D) GSK3β. (E) RUNX-2. (F) BMP-2. *, P < 0.05; **, P < 0.01.

Article Snippet: ICA and magnesium chloride were added separately to the complete medium of BMSCs (Cyagen, China).

Techniques: Expressing

Journal: Biomaterials Research

Article Title: Repair of Osteoporotic Bone Defects in Rats via the Sirtuin 1-Wnt/β-catenin Signaling Pathway by Novel Icariin/Porous Magnesium Alloy Scaffolds

doi: 10.34133/bmr.0090

Figure Lengend Snippet: ICA and Mg 2+ act synergistically to promote the expression of osteogenic differentiation-related proteins in rat BMSCs via the SIRT1-Wnt/β-catenin signaling pathway. (A) Results of Western blot experiment. (B) Statistical graph of Western blot experiment results. *, P < 0.05; **, P < 0.01.

Article Snippet: ICA and magnesium chloride were added separately to the complete medium of BMSCs (Cyagen, China).

Techniques: Expressing, Western Blot

Journal: Biomaterials Research

Article Title: Repair of Osteoporotic Bone Defects in Rats via the Sirtuin 1-Wnt/β-catenin Signaling Pathway by Novel Icariin/Porous Magnesium Alloy Scaffolds

doi: 10.34133/bmr.0090

Figure Lengend Snippet: SEM and bulk sample observation of the surface structure of different scaffolds and biosafety of the porous magnesium alloy scaffold. (A) Porous magnesium alloy scaffold and novel ICA/porous magnesium alloy scaffold. (B) Confocal microscopic observation of BMSCs. (C) SEM observation of BMSCs and (D) visceral HE staining after implantation of the porous magnesium alloy scaffold in rats.

Article Snippet: ICA and magnesium chloride were added separately to the complete medium of BMSCs (Cyagen, China).

Techniques: Staining